Exchange an old EP HPLC method of 160 min analysis time against a better and faster method. Separate the drug substance from all the impurities with a minimum resolution of 2.0. The limit of the …

Temperature: 30 °C Mobile Phase: A: 20 mM Phosphate buffer, B: Acetonitrile. Temperature: 30°C. Detection: UV 245 nm. Sample: Acetaminophen impurities. Expected dwell volume in UHPLCs – μL …

UPLC stands as a potent analytical technique, offering speed, efficiency, and sensitivity in compound separations. This introductory guide provides insights into its principles, benefits, instrumentation, …

The ACQUITY UPLC columns calculator estimates the plate count (N) of an isocratic separation or the peak capacity (Pc) of a gradient separation based on your current HPLC conditions.

Inlet (LC) method file: In this file you will specify the overall LCMS runtime (the length of your LC elution program), the mobile phase composition as a function of time (isocratic or gradient), the autosampler …

UPLC is utilized for smaller particles < 2 μm in diameter. It is highly sensitive, dynamic, and efficient technique with improved resolution, requiring lesser use of solvents and give faster results, making …

Nov 24, 2023 · I am developing a UHPLC method for content and purity for a peptide based on 100mM KPF6 and 100mM NH4PF6 pH 3.5. TFA as an ion pair reagent does not give the required separation …

Change the time of Pre-Inject Wash and Post-Inject Wash from the General tab of the ACQ-FTN instrument method. First, increase the time for Post-Inject Wash, and if this doesn't help, enter the …

Apr 5, 2025 · UPLC plays a crucial role in drug metabolism studies, stability assessments, and bioactive compound quantification following in vitro experiments.

Solve complex UPLC/UHPLC separation challenges with the ACQUITY UPLC Systems with 2D LC Technology, a family of systems and proven columns that offer the lowest dispersion for maximum …